Description
Summary:
Provides the methods used for general cell culture expansion and maintenance. Specific protocols may be prepared as needed for particular cell lines. Covers cell coutning using a hemacytometer, inoculating (seeding) and scale-up (splitting) of cell cultures, harvest of adherent (attachment dependent) cell lines, remove of cells by Trypsin or Trypsin-EDTA, removal by EDTA, removal by scraping, maintenance of attachment-dependent cell lines, maintenance of non-attachment-dependent cell lines, and cleanup. Also includes description of indications of instability or deterrioration of cell cultures.
Included Sections:
Purpose, Scope, Responsibility, References and Applicable Documents, Materials and Equipment, Health and Safety Considerations, Documentation Requirements, and Procedures
Included Attachments/Appendices:
Generic Cell Line Scale Up and Maintenance Form
About This Document:
This is not a generic template, it's a 9-page procedure that was actually created and used in the Bioprocessing operations of a FDA-regulated establishment. The company-specific information (names, addresses, some images, proprietary product information, etc.) has been redacted but the content and format have been largely preserved.
Reasons to Buy:
- Use it as a starting point for your own documentation project
- Compare the quality of your documents against your industry peers
- Learn what other companies in your industry are actually doing
Who Will Benefit:
- Biologic Manufacturing Personnel
- Process Development Personnel
- Method Development Personnel
Product Details
- Edition:
- 1.0
- Published:
- 08/01/2007
- Number of Pages:
- 9
- File Size:
- 1 file , 450 KB
