Description
Summary:
Describes a method for determining the content of colony forming units-megakaryocyte (CFU-MK) by plating a known number of hematopoietic cells in a dish and enumerating the number of colonies generated after 14 days. Applies to the set-up and enumeration of CFU-MK. Applies to assays performed by R&D personnel. Includes instructions for reagent preparation, plate setup, fixing, immunocytochemistry staining, and CFU-MK enumeration.
Included Sections:
Purpose, Scope, Responsibility, References and Applicable Documents, Materials and Equipment, Health and Safety Considerations, Documentation Requirements, and Procedures
Included Attachments/Appendices:
CFU-Mk Reagent Preparation Worksheet, CFU-Mk Preparation Worksheet, CFU-Mk Staining Worksheet
About This Document:
This is not a generic template, it's a 10-page procedure that was actually created and used in the Laboratory R&D operations of a FDA-regulated establishment. The company-specific information (names, addresses, some images, proprietary product information, etc.) has been redacted but the content and format have been largely preserved.
Reasons to Buy:
- Use it as a starting point for your own documentation project
- Compare the quality of your documents against your industry peers
- Learn what other companies in your industry are actually doing
Who Will Benefit:
- Process Development Personnel
- Method Development Personnel
- Quality Control Personnel
Product Details
- Edition:
- 1.0
- Published:
- 08/01/2007
- Number of Pages:
- 10
- File Size:
- 1 file , 530 KB
